This species is diagnosed from other species of Glanidium, except G. albescens, by the presence of small dark spots over a pale yellow ground color (vs. large dark blotches over a pale brown ground color). It differs from G. albescens by having pectoral fin spine length, about 21-26 % in SL (vs. 17-20% in SL), 10 ribs (vs. 9) and 36-37 vertebrae (vs. 38-39). The possession of 36 or 37 vertebrae separates this species from G. cesarpintoi, G. albescens, G. leopardum, G. melanopterum, G. catharinensis, and G. ribeiroi (with 38 or 39 in G. cesarpintoi, 38 or 39 in G. albescens, 40 or 41 in G. leopardum, 41 in G. melanopterum, 41 in G. catharinensis, and 44 in G. ribeiroi). It also differs from other species by having anterior margin of the pectoral-fin spine with serrae on its entire margin (vs. serrae restricted to distal portion of spine in G. melanopterum, G. catharinensis and G. ribeiroi or smooth anterior margin in G. leopardum) and by a long sharpened ventral process on urohyal (vs distinctly short robust process in G. melanopterum, G. cesarpintoi, G. catharinensis, and G. ribeiroi) (Ref. 93811).